During the MLS SuperDraft 2025, teams selected 90 players from college soccer and other avenues.
- Round 2: Ronan Wynne (No. 53) - D, Denver
- Round 3: William Kulvik (No. 83) - D, Maryland
- Round 2: Riley Thomas (No. 42) - D, UNC Chapel Hill
- Round 3: Patrick Gryczewski (No. 72) - F, Rhode Island
- Round 1: Mikah Thomas (No. 9) - D, Connecticut
- Round 2: Andrew Johnson (No. 47) - D, Cornell
- Round 3: Barzee Blama (No. 77) - F, Mercer
- Round 1: Dean Boltz (No. 3) - F, Wisconsin
- Round 2: Travis Smith Jr. (No. 33) - D, Wake Forest
- Round 3: Geni Kanyane (No. 63) - D, Dayton
- Round 3: Ben Augee (No. 80) - M, Gonzaga
Colorado Rapids
- Round 1: Alex Harris (No. 4) - F, Cornell
- Round 1: Matthew Senanou (No. 6) - D, Xavier
- Round 1: Efetobo Aror (No. 12) - M, Portland
- Round 1: Sydney Wathuta (No. 16) - F, Vermont
- Round 1: Joshua Copeland (No. 26) - F, Detroit Mercy
- Round 2: Charlie Harper (No. 38) - D, UNC Chapel Hill
- Round 3: Donovan Phillip (No. 62) - F, North Carolina State
- Round 3: Shawn Smart (No. 68) - D, Las Vegas Lights
- Round 3: Matthew Van Horn (No. 75) - M, Georgetown
- Round 2: Cesar Ruvalcaba (No. 51) - D, Southern Methodist
- Round 3: Drew Kerr (No. 81) - F, Duke
FC Dallas
- Round 1: Enzo Newman (No. 11) - D, Oregon State
- Round 2: Samuel Sarver (No. 41) - F, Indiana
- Round 3: Mohammed Cisset (No. 71) - D, Penn State
D.C. United
- Round 1: Hakim Karamoko (No. 10) - F, North Carolina State
- Round 2: Daniel Ittycheria (No. 40) - F, Princeton
- Round 3: Jonah Biggar (No. 70) - D, South Carolina
- Round 3: Grant Bailey (No. 86) - D, Loyola Chicago
Houston Dynamo FC
- Round 2: Bo Cummins (No. 48) - D, Wake Forest
- Round 1: Alec Hughes (No. 22) - F, Massachusetts
- Round 1: Jason Bucknor (No. 20) - D, Michigan
- Round 2: Jacob Woznicki (No. 60) - F, Hofstra
- Round 3: Nicklaus Sullivan (No. 90) - D, LAFC II
Inter Miami CF
- Round 2: Bailey Sparks (No. 52) - M, Southern Methodist
- Round 3: Michael Appiah (No. 82) - F, Florida International
Minnesota United FC
- Round 1: Roman Torres (No. 25) - M, Creighton
- Round 2: Kieran Chandler (No. 43) - D, Connecticut
- Round 2: Logan Dorsey (No. 55) - F, Kentucky
- Round 3: Nick Collins (No. 85) - D, Rutgers
- Round 1: Michael Adedokun (No. 13) - F, Ohio State
- Round 2: Arik Duncan (No. 44) - F, Califonia, Berkeley
- Round 2: Caden Grabfelder (No. 50) - F, Penn State
- Round 3: Arthur Duquenne (No. 73) - D, Clemson
Nashville SC
- Round 2: Alioune Ka (No. 36) - F, Cornell
- Round 3: Ethan Ballek (No. 66) - F, South Carolina
- Round 1: Donovan Parisian (No. 18) - GK, San Diego
- Round 2: Eric Howard (No. 35) - D, Georgetown
- Round 3: CJ Williams (No. 65) - D, Boston College
- Round 1: Max Murray (No. 17) - D, Vermont
- Round 2: Collin McCamy (No. 54) - M, Northwestern University
- Round 3: AJ Smith (No. 78) - F, Virginia
- Round 3: Arnau Farnos (No. 84) - F, Oregon State
- Round 3: Nicholas Cavallo (No. 88) - D, UCLA
New York Red Bulls
- None
Orlando City SC
- Round 1: Joran Gerbet (No. 27) - M, Clemson
- Round 2: Titus Sandy Jr. (No. 46) - D, Clemson
- Round 2: Collins Oduro (No. 57) - F, Indiana
- Round 3: Takahiro Fujita (No. 87) - D, Marshall
Philadelphia Union
- None
Portland Timbers
- Round 1: Ian Smith (No. 14) - D, Denver
- Round 3: Lukas Burns (No. 74) - GK, Providence
Real Salt Lake
- Round 1: Jesus Barea (No. 19) - F, Missouri State
- Round 1: Max Kerkvliet (No. 21) - GK, Connecticut
- Round 1: Lineker Rodrigues Dos Santos (No. 29) - F, Marshall
- Round 1: Sergi Solans (No. 30) - M, Oregon State
- Round 2: Nick Dang (No. 49) - D, Virginia
- Round 2: Trace Alphin (No. 59) - GK, Wake Forest
- Round 3: Liam O'Gara (No. 79) - M, Wake Forest
- Round 3: Aleksandar Vukovic (No. 89) - D, Marshall
- Round 1: Manu Duah (No. 1) - M, UC Santa Barbara
- Round 1: Ian Pilcher (No. 24) - D, UNC Charlotte
- Round 2: Harrison Bertos (No. 31) - D, Washington
- Round 2: Samy Kanaan (No. 56) - F, San Diego
- Round 3: Donovan Sessoms (No. 61) - F, Sacramento State
- Round 1: Max Floriani (No. 2) - D, Saint Louis
- Round 1: Reid Roberts (No. 5) - D, San Francisco
- Round 2: Nick Fernandez (No. 32) - M, Portland
- Round 1: Ryan Baer (No. 28) - M, West Virginia
- Round 2: Demian Alvarez (No. 58) - D, Seattle
- Round 3: Trace Terry (No. 76) - F, Bowling Green State
Sporting Kansas City
- Round 1: Jansen Miller (No. 8) - D, Indiana
- Round 2: Anthony Samways (No. 34) - D, Ohio State
- Round 3: Leon Koehl (No. 64) - M, Maryland
- Round 1: Emil Jaaskelainen (No. 7) - F, Akron
- Round 2: Joey Zalinsky (No. 37) - D, Rutgers
- Round 3: Colin Welsh (No. 67) - GK, Western Michigan
Toronto FC
- Round 1: Reid Fisher (No. 23) - D, San Diego State
- Round 2: Michael Sullivan (No. 39) - M, Pittsburgh
- Round 3: Joseph Melto Quiah (No. 69) - F, Dayton
Vancouver Whitecaps FC
- Round 1: Tate Johnson (No. 15) - D, UNC Chapel Hill
- Round 2: Nikola Djordjevic (No. 45) - D, Southern Methodist